What is Keratinase Subtilisin CAS 9014-01-1?

Keratinase (Subtilisin), CAS 9014-01-1, is a serine protease enzyme that can hydrolyze keratin-containing proteins such as feathers and hair. It is produced by microbial fermentation and is also known as subtilisin, alkaline protease, or keratinolytic protease.

What pH and temperature are best for Keratinase?

Industrial keratinase/subtilisin grades usually work best in neutral to alkaline conditions, often around pH 7.0-10.5, and at temperatures of roughly 40-60 C. Exact optima vary by strain, formulation, and substrate.

Can Keratinase replace sodium sulfide in leather unhairing?

It can reduce or partially replace sodium sulfide by selectively attacking keratin at the hair root while preserving collagen. The exact replacement level depends on hide type and process conditions, so it is usually optimized within a lower-sulfide unhairing system.

What are the main industrial uses of Keratinase?

Common uses include leather unhairing, hydrolysis of feathers and keratin-rich by-products, animal-feed protein digestibility, and alkaline protease applications in cleaning or waste processing. The right activity and formulation depend on the target substrate.

Keratinase (Subtilisin) CAS 9014-01-1

Keratinase, also called Subtilisin or keratinolytic protease, CAS 9014-01-1, is a serine protease enzyme supplied for feed and industrial applications. Confirm the required activity, grade and specification, then request a quotation with COA/TDS/SDS for bulk supply.

CAS: 9014-01-1
Synonyms: Subtilisin, Keratinolytic protease, Bacillus subtilis protease, Endopeptidase K, Proteinase
Einecs: 232-752-2
Molecular Formula: Not applicable
Grade: feed additives
Package: 25kg/bag (Plastic woven bag with PE liner) or 500kg/1000kg Jumbo Bag.

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Product Description

Keratinase CAS 9014-01-1 is a serine protease enzyme, also known as Subtilisin, that hydrolyzes keratin-containing proteins such as feathers, hair and wool, with EINECS 232-752-2.

Synonyms: Subtilisin; Keratinolytic protease; Bacillus subtilis protease; Endopeptidase K; Proteinase.

CAS: 9014-01-1.

As an enzyme protein, it has no fixed molecular formula; activity is specified by enzyme units.

It is supplied as a powder or liquid enzyme preparation.

Industrial grades usually work best in neutral to alkaline conditions, often around pH 7.0–10.5.

Standard enzyme powders or liquids can lose activity above about 60–70 °C.

For high-temperature feed conditioning and pelleting, heat-stable or coated grades are recommended.

It can reduce or partially replace sodium sulfide in leather unhairing by attacking keratin at the hair root while preserving collagen.

It hydrolyzes feathers and keratin-rich by-products into more digestible peptides and amino acids.

In animal feed it improves protein digestibility and nutrient utilization.

It is also used in selected detergent, textile and waste-processing applications.

Store in a cool, dry, well-ventilated area away from excessive heat and moisture to preserve enzyme activity.

Protect from direct sunlight and avoid strong acids, alkalis and oxidizers.

Confirm appearance, enzyme activity, pH range, moisture and microbial limits as required.

Use dust masks or respirators and eye protection during handling, and wash skin contact with soap and water.

Confirm packaging with the requested quantity and destination requirements.

The page is an RFQ entry point and does not state stock, price, MOQ or fixed lead time.

For an RFQ, state the intended use, required activity, grade and specification, quantity, packaging, destination and document needs.

FAQ - Technical & Supply Chain

Q:
What is Keratinase Subtilisin CAS 9014-01-1?

A: Keratinase (Subtilisin), CAS 9014-01-1, is a serine protease enzyme that can hydrolyze keratin-containing proteins such as feathers and hair. It is produced by microbial fermentation and is also known as subtilisin, alkaline protease, or keratinolytic protease.
Q:
What pH and temperature are best for Keratinase?

A: Industrial keratinase/subtilisin grades usually work best in neutral to alkaline conditions, often around pH 7.0-10.5, and at temperatures of roughly 40-60 C. Exact optima vary by strain, formulation, and substrate.
Q:
Does Keratinase remain active during feed pelleting?

A: Standard enzyme powders or liquids can lose activity above about 60-70 C. For high-temperature feed conditioning and pelleting, heat-stable or microencapsulated grades are usually selected to survive the thermal step.
Q:
Can Keratinase replace sodium sulfide in leather unhairing?

A: It can reduce or partially replace sodium sulfide by selectively attacking keratin at the hair root while preserving collagen. The exact replacement level depends on hide type and process conditions, so it is usually optimized within a lower-sulfide unhairing system.
Q:
What are the main industrial uses of Keratinase?

A: Common uses include leather unhairing, hydrolysis of feathers and keratin-rich by-products, animal-feed protein digestibility, and alkaline protease applications in cleaning or waste processing. The right activity and formulation depend on the target substrate.

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