Sephadex G-10 CAS 9050-94-6
Release time: 2025-07-25
Physicochemical properties
| Property | Description |
|---|---|
| Chemical Name | Sephadex G10 (Dextran Gel G10) |
| CAS Number | 9050-68-4 |
| Molecular Formula | C₁₃H₁₀BrN₃O₂ |
| Appearance | White or off-white spherical beads (dry form); swells to form a gel in aqueous solutions |
| Density | 1.7 ± 0.1 g/cm³ |
| Boiling Point | 461.1 ± 25.0 °C at 760 mmHg (Note: This value is likely for a specific component or impurity; dextran gels decompose before boiling) |
| Flash Point | 232.7 ± 23.2 °C |
| Refractive Index | 1.721 |
| Solubility | Insoluble in water and organic solvents; swells in water to form a gel |
| pH Stability | Stable over a wide pH range (typically pH 2-13) |
| Particle Size Range | 40-120 μm (common grade); other grades available (e.g., 200-500 μm) |
| Fractionation Range | Globular proteins: < 700 Da; linear molecules: < 700 Da (minimum exclusion limit) |
| Application | Gel filtration chromatography for desalting, buffer exchange, and purification of small biomolecules (peptides, small proteins, oligosaccharides) |
| Storage Conditions | Store at 4-30 °C in a dry, well-ventilated area; avoid exposure to moisture and direct sunlight |
Application Areas
Separation and purification: used for the separation and purification of peptides, proteins (molecular weight>700), and cephalosporin drugs.
Desalination and buffer replacement: Quickly remove salt, dye, and radiolabeled contaminants, suitable for small molecule biological samples.
Antibiotic analysis: detecting high molecular weight polymers of antibiotics such as cefoperazone sodium and penicillin
Storage conditions
Storage medium: It should be sealed with a 20% ethanol aqueous solution to prevent microbial growth and water evaporation.
Temperature control: It is recommended to refrigerate at 4-8 ℃ (freezing is prohibited), and store at room temperature with strict sealing.
Avoid oxidants: long-term contact with oxidants will damage the gel structure, so keep away from such substances
Safety precautions
Operating specifications: pH range: 2-13, avoid strong acids and bases (concentration<0.15 moles). Do not backwash in reverse to prevent damage to the column tail sieve plate.
Personal protection: It is recommended to wear goggles, gloves, and N95 masks during operation.
Sample processing: Filter with a 0.45 μ m filter membrane before use to avoid pigment or impurity clogging of column 4.
Swelling treatment: dry powder needs to be soaked in distilled water for 24 hours or swelled with hot water (not boiling) to remove floating gel fragments
